kasumi-1, 人白血病细胞株产品信息
订购信息
| 货号 |
细胞名称 |
种属 |
价格 |
到货周期 |
| CC029 |
kasumi-1, 人白血病细胞株 |
Human |
询价
|
现货 |
培养条件
RPMI-1640(添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)，80%；优质胎牛血清，20%
气相：空气 ，95%；二氧化碳，5%
温度：37摄氏度
细胞描述
细胞生长：悬浮生长
细胞形态：成髓细胞样
细胞数量：1× 106个
细胞传代：维持细胞浓度在3×105~3×106cells/mL，每周补液2~3次
细胞特性：The cell line was established from the peripheral blood of an acute myeloid leukemia (AML) patient.
This is a leukemic cell line with an 8;21 chromosome translocation. This translocation juxtaposes the AML1 with ETO (or MTG8) gene, giving rise to the fusion gene AML1-ETO (also known as AML1-MTG or RUNX1-CBF2T1), hence the cells produce chimeric AML1-ETO protein. This protein down-regulates CEBPA mRNA, protein and DNA binding activity, which is crucial for the differentiation of granulocytes.
The cells are positive for myeloperoxidase showing a morphology of myeloid maturation. In proliferation assay the cells in culture showed response to interleukin-3 (IL-3), IL-6, granulocyte colony-stimulating factor (G-CSF), and granulocytemacrophage CSF (GM-CSF), but not to IL-1 or IL-5.
Neither granulocytic nor eosinophilic maturation was observed in the in vitro liquid culture by the addition of dimethyl sulfoxide, G-CSF, or IL-5, respectively. Induction of macrophagelike cells was seen by the addition of phorbol ester. Proliferation is inhibited by 1,25S-(OH)2-16,23-diene-26-F3-10-nor D3.
细胞纯度：93％
细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支 原体、细菌、酵母和真菌
细胞冻存：液氮冻存（基础培养基+10%DMSO+20%FBS）
细胞运输： 干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗
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