大鼠淋巴成纤维细胞产品信息
订购信息
| 货号 |
细胞名称 |
种属 |
价格 |
到货周期 |
| PC024 |
大鼠淋巴成纤维细胞 |
Rattus |
询价
|
4-5 周 |
培养条件
成纤维细胞培养基（Fibroblast Medium）
气相：空气，95%；二氧化碳，5%
温度：37摄氏度
细胞描述
细胞生长：贴壁生长
细胞形态：成纤维细胞样
细胞数量：1×106个
细胞传代：1:3传代；2~3天换液1次
细胞特性：Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen [2]. Besides, it has been reported that the growth of splenic fibroblasts has increased in lymphoproliferatived diseases [3].
RLF from ScienCell Research Laboratories are isolated from rat lymph nodes. RLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. RLF are negative mycoplasma, bacteria, yeast and fungi. RLF are guaranteed to further expand for 5 population doublings at the condition provided by ScienCell Research Laboratories.
细胞纯度：93％
细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存：液氮冻存（基础培养基+10%DMSO+20%FBS）
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗
Reference
[1] Conrad, G. W., Hart, G. W., Chen, Y. （1977） Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration （L. E. Glynn, ed.）, pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
[3] Domracheva, E. V., Rogova, E. M., Vorob'ev, A. L. （1984） Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.
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