启动子产品信息
订购信息
货号 |
种属 |
载体 |
描述 |
价格 |
周期 |
PR10370 |
Mouse |
pGL3-basic |
|
询价
|
3-4 周 |
产品参数
Gene Promoter: Tyrosinase
Specificity: Melanocytes & melanoma
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.
产品说明书
请联系YRgene技术支持索取产品说明书。
产品说明
Description:
Tyrosinase, the rate-limiting enzyme in melanin synthesis, is expressed specifically in pigment-producing cells. Expression of tyrosinase is regulated by cyclic AMP (cAMP). The M-box 70-bp upstream from the TATA-box and the E-box located downstream the TATA-box, near the initiator site, are involved in the regulation of the tyrosinase promoter activity by cAMP [1]. Melanoma-specific expression of a transgene can be achieved by using the tyrosinase promoter [2], this targeted expression being further amplified by addition of the melanocyte-specific enhancer.
1. Bertolotto C. et al. 1996. Regulation of tyrosinase gene expression by cAMP in B16 melanoma cells involves two CATGTG motifs surrounding the TATA box: implication of the microphthalmia gene product. J Cell Biol. 134(3):747-55. 2. Vile R. et al. 1994. Acomparison of the properties of different retroviral vectors containing the murine tyrosinase promoter to achieve transcriptionally targeted expression of the HSVtk or IL-2 genes. Gene Ther. 1(5):307-16.
立即购买
加入购物车