启动子产品信息
订购信息
| 货号 |
种属 |
载体 |
描述 |
价格 |
周期 |
| PR10270 |
Human |
pGL3-basic |
|
询价
|
3-4 周 |
产品参数
Gene Promoter: Surfactant Protein B
Specificity: Lung
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.
产品说明书
pDRIVE-SPB(human) Map 产品说明书(点击下载)
pDRIVE5-SEAP-SPB(human) Map 产品说明书(点击下载)
pDRIVE5-SEAP-SPB(human) Sequence 产品说明书(点击下载)
产品说明
Description:
Surfactant protein B (SP-B) is a developmentally and hormonally regulated lung protein that is required for normal surfactant function. SP-B is selectively expressed in bronchiolar and alveolar epithelial cells of the lung. The human SP-B promoter fragment directs tissue- and lung cell-specific transgene expression [1]. It contains two cis-acting elements for two transcription factors, thyroid transcription factor (TTF)-1 and hepatocyte nuclear factor (HNF)-3 that are important for the specificity and the activation of gene expression in the lung [2]. Promoter activity is strongly inhibited in a dosedependent fashion by phorbol ester, such as 12-O-tetradecanoylphorbol-13-acetate (TPA).
1. Strayer M. et al. 2002. Human surfactant protein B promoter in transgenic mice: temporal, spatial, and stimulus-responsive regulation. Am J Physiol Lung Cell Mol Physiol 282(3):L394-404.
2. Kumar AS. et al., 1997. Phorbol ester down-regulation of lung surfactant protein B gene expression by cytoplasmic trapping of thyroid transcription factor-1 and hepatocyte nuclear factor 3. J Biol Chem. 72(33):20764-73.
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