启动子产品信息
订购信息
| 货号 |
种属 |
载体 |
描述 |
价格 |
周期 |
| PR10256 |
Human |
pDrive |
|
询价
|
3-4 周 |
产品参数
Gene Promoter: Nephrin
Specificity: Podocytes
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, ect.
产品说明书
pDRIVE-NphsI(human) Map 产品说明书(点击下载)
pDRIVE-NphsI(human) Sequence 产品说明书(点击下载)
产品说明
Description:
Nephrin is a transmembrane protein that belongs to the immunoglobulin superfamily and is localized to the slit diaphragms that are a critical component of the glomerular filtration barrier in the kidney. A 1.25 kb fragment of the nephrin gene promoter, which includes the initiation codon and immediate 5’-flanking region, is capable of directing podocyte-specific expression of a β-galactosidase transgene in vivo [1,2]. This promoter lacks a TATA box but contains GATA binding sites and E-box consensus sequences. An enhancer was identified within the proximal promoter which is activated by the Wilms tumor suppressor protein (WT1) in vitro [3].
1. Wong MA. et al., 2000. Identification and characterization of a glomerular-specific promoter from the human nephrin gene. Am J Physiol Renal Physiol. 279(6):F1027-32.
2. Moeller MJ. et al., 2002. Two gene fragments that direct podocyte-specific expression in transgenic mice. J Am Soc Nephrol. 13(6):1561-7.
3. Guo G. et al., 2004. WT1 activates a glomerular-specific enhancer identified from the human nephrin gene. J Am Soc Nephrol. 15(11):2851-6.
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