启动子产品信息
订购信息
| 货号 |
种属 |
载体 |
描述 |
价格 |
周期 |
| PR10135 |
Mouse |
pUC57 |
|
询价
|
2-3 周 |
产品参数
Gene Promoter: PGK-1
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP, pGL3-basic, pUC57, pcDNA3.1-EGFP, ect.
产品说明书
pDRIVE-PGK-1(mouse) Map 产品说明书(点击下载)
pDRIVE-PGK-1(mouse) Sequence 产品说明书(点击下载)
pDRIVE5-SEAP-PGK-1(mouse) Map 产品说明书(点击下载)
pDRIVE5-SEAP-PGK-1(mouse) Sequence 产品说明书(点击下载)
产品说明
Description:
Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, a potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box [1]. This promoter can efficiently drive high levels of expression of reporter genes (i.e. LacZ and GFP) and therapeutic genes, such as tumor-associated antigenes [2, 3]. Furthermore, in contrast to the CMV promoter, the PGK promoter yields sustained expression [2].
1. Adra CN. et al. 1987. Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Gene 60(1):65-74.
2. Gerolami R. et al. 2000. Gene transfer to hepatocellular carcinoma: transduction efficacy and transgene expression kinetics by using retroviral and lentiviral vectors. Cancer Gene Ther 7(9):1286-92.
3. Lizee G. et al., 2004. Lentivirus vector-mediated expression of tumor-associated epitopes by human antigen presenting cells. Hum Gene Ther. 15(4):393-404.
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