pTet-On产品信息
订购信息
货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
VSC0460 |
pTet-On |
Clontech |
四环素调控系统
|
询价
|
现货 |
载体描述
pTet-On expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. pTet-On can be distinguished from pTet-Off by digestion with Hind III.
产品说明书
pTet-On 载体说明 产品说明书(点击下载)
载体序列
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