pBridge产品信息
订购信息
货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
VJC0495 |
pBridge |
Clontech |
酵母三杂交系统
|
询价
|
现货 |
载体描述
pBridge expresses two proteins: a DNA-binding domain fusion, and an additional protein. pBridge thus allows establishment of three-hybrid systems when used in combination with an activation domain fusion vector and yeast strains from any of Clontech's GAL4-based two-hybrid systems, including Matchmaker? Gold. This vector generates a hybrid protein that contains the sequences for the GAL4 DNA-binding domain (DNA-BD) and the sequence cloned into MCS I. The fusion protein is expressed in yeast host cells from the constitutive ADH1 promoter; transcription is terminated at the ADH1 transcription termination signal. The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences (NLS) that are an intrinsic part of the GAL4 DNA-BD. An additional gene of interest can be cloned into MCS II which is located downstream of an HA epitope and a second NLS. The resulting fusion protein is conditionally expressed from the Met25 promoter in response to methionine levels in the medium; i.e., it is repressed in the presence of 1 mM methionine and expressed in the absence of methionine.
产品说明书
pBridge 载体说明 产品说明书(点击下载)
pBridge 载体序列 产品说明书(点击下载)
载体序列
请联系YRgene技术支持索取产品载体序列。
质粒图谱
立即购买
加入购物车