YEP352产品信息
订购信息
货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
VJA0316 |
YEP352 |
ATCC |
酵母表达载体
|
询价
|
1-2 周 |
载体描述
Constructed by filling in and blunt-end ligating pUC18 linearized at NdeI, a 1.4 kb RsaI fragment of the 2 micron circle, and a 1.1 kb HindIII/SmaI fragment containing URA3. A XbaI site in 2u and a PstI site in URA3 were removed. The yeast genomic sequence is in nt 286-1383 (where the A of the ATG of lacZ' is number 1), the 2 micron sequence goes from nt 1384-2768, & pUC18 includes nt 2769-285. Restriction digests of the clone give the following sizes (kb): BamHI--5.3; XbaI--5.3; PstI--5.3; HindIII--5.3. (ATCC staff) This is a E.coli/yeast episomal shuttle vector with multiple restriction sites for cloning. (personal communication) Medium is 1227 LB plus ampicillin. NCBI gi: 295271 Hosts: E.coli RR1, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pBR322, 2micron.
产品说明书
YEP352 载体序列 产品说明书(点击下载)
载体序列
请联系YRgene技术支持索取产品载体序列。
质粒图谱
立即购买
加入购物车