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VRC0347	RNAi-Ready pSIREN-Retro Q-ZsGreen	Clontech	RNAiÔØÌå	ѯ¼Û	ÏÖ»õ

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Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic¡ªampicillin
Replication: prokaryotic¡ªCol E1 ori (low copy), viral¡ªSV40 ori

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RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.

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