pEF1/myc-His C产品信息
订购信息
| 货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
| VPI0028 |
pEF1/myc-His C |
Invitrogen |
哺乳动物表达载体
|
询价
|
现货 |
产品参数
Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag (6x His)
Constitutive or Inducible System：Constitutive
载体描述
pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
- Human elongation factor 1?-subunit (hEF-1α) promoter for high-level expression across a broad range of species and cell types (Goldman et al., 1996; Mizushima and Nagata, 1990).
- Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc (c-myc) epitope and a polyhistidine metal-binding peptide.
- Neomycin resistance gene for selection of stable cell lines.
- Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS7).
The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
产品说明书
pEF1/myc-His A&B&C 载体图谱 产品说明书(点击下载)
pEF1/myc-His A&B&C 载体说明 产品说明书(点击下载)
载体序列
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