pUB6/V5 His C产品信息
订购信息
货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
VPI0024 |
pUB6/V5 His C |
Invitrogen |
哺乳动物表达载体
|
询价
|
现货 |
产品参数
Key Function:Constitutive Expression
Mammalian Selection:Blasticidin
Promoter:UbC
Vector Type:pUB6
Cloning Method:Restriction Enzyme/MCS
Protein Tag or Fusion:V5 Epitope Tag,His Tag (6x His)
Constitutive or Inducible System:Constitutive
载体描述
pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.
The vectors contain the following elements:
- Human ubiquitin C promoter (hUbC) for high-level expression across a broad range of species and cell types (Schorpp et al., 1996; Wulff et al., 1990).
- Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine (6×His) metal-binding tag.
- Blasticidin resistance gene (bsd) for selection of stable cell lines.
- Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7).
The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
产品说明书
pUB6/V5-His A&B&C 载体图谱 产品说明书(点击下载)
pUB6/V5-His A&B&C 载体说明 产品说明书(点击下载)
载体序列
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