这是一个双表达框载体，CMV启动子后有MCS供插入外源基因，hEF1α启动子调控Cycle 3 GFP-Zeocin? fusion的表达，可以做到既有荧光蛋白来做转染指示，且荧光基因表达和外源基因表达又不会互相干扰，而且Zeocin也可以用来做稳定性细胞筛选。另外，由于EM-7启动子的存在，Cycle 3 GFP-Zeocin? fusion在E.coli中也可以表达，可以用来做原核荧光定位。
Three pTracer? mammalian expression vectors are available that express GFP fused to the selectable marker Zeocin? . Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Zeocin? fusion.
All three pTracer? vectors have the following features:
- Cycle 3 GFP-Zeocin? fusion for selection in mammalian cell lines
- BGH polyA signal and transcription termination sequences to enhance mRNA stability
The pTracer?-SV40 vector offers:
- SV40 promoter for high-level constitutive expression of your gene of interest
- CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
- SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen
The pTracer?-CMV2 vector offers:
- CMV promoter for high-level constitutive expression of your gene of interest
- EF-1α promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
- Ampicillin resistance gene for selection in E. coli
The pTracer?-EF vectors offer:
- EF-1α promoter for high-level constitutive expression of your gene of interest
- CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin? fusion
- C-terminal V5 epitope tag for simple detection of recombinant fusion proteins with an Anti-V5 Antibody
- C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
- Multiple cloning site supplied in three reading frames to simplify cloning in frame with the C-terminal tag
