pIRES-hyg3产品信息
订购信息
货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
VPC0049 |
pIRES-hyg3 |
Clontech |
哺乳动物表达载体
|
询价
|
现货 |
产品参数
Key Function:Constitutive Expression
Mammalian Selection:Neo,G418
Promoter:CMV
Cloning Method:Restriction Enzyme/MCS
Constitutive or Inducible System:Constitutive
载体描述
IRES Bicistronic Expression Vectors allow you to rapidly and efficiently select positive clones that express your target protein. They include a single cassette that expresses both your gene of interest and a selection marker or reporter gene from a single promoter, so that virtually all transfected cells expressing the selection marker also express your gene of interest. With these vectors, you can screen fewer colonies to locate clones expressing high levels of your protein.
The pIRES Vectors contain the internal ribosome entry site (IRES) of the encephalomyocarditis virus (ECMV), which permits the translation of two open reading frames from one messenger RNA. Ribosomes can enter the bicistronic mRNA either at the 5' end to translate the gene of interest, or at the ECMV IRES to translate the selection marker or a reporter gene. All IRES Selection Vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene, and allows preferential selection of cells expressing high levels of your protein of interest.
产品说明书
pIRES-hyg3 载体序列 产品说明书(点击下载)
pIRES-hyg3 载体说明书 产品说明书(点击下载)
载体序列
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