pMIB v5-His B产品信息
订购信息
| 货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
| VKI0141 |
pMIB v5-His B |
Invitrogen |
昆虫细胞表达载体
|
询价
|
现货 |
产品参数
Mammalian Selection：Blasticidin
Promoter：OplE2
Vector Type：pMIB
Cloning Method：Restriction Enzyme/MCS
Protein Tag or Fusion：V5 Epitope Tag，His Tag (6x His)
Protein Tag Position (to your gene)：C-terminal
测序引物：
| Primer |
Sequence |
pMoles Supplied |
| OpIE2 |
Forward 5′-CGCAACGATCTGGTAAACAC-3′ |
329 |
| OpIE2 |
Reverse 5′-GACAATACAAACTAAGATTTAGTCAG-3′ |
250 |
载体描述
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five? insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
- The OpIE2 promoter for constitutive expression
- The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
- The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
- C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
产品说明书
pMIB/V5-His A&B&C 产品说明书(点击下载)
pMIB/V5-His A&B&C 产品说明书(点击下载)
载体序列
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