pJQ200SK产品信息
订购信息
| 货号 |
载体名称 |
出品公司 |
载体用途 |
价格 |
到货周期 |
| VJA0527 |
pJQ200SK |
ATCC |
基因置换载体（自杀型）
|
询价
|
现货 |
产品参数
Size: 5400
Promoters: Promoter lac
Construction: P15A ori, sacB, RP4, gtmR, pBluescriptSK MCS
Marker(s):sacB,gtmR
载体描述
Suicide vector permitting gene replacement and mobilization into a wide range of Gram negative bacteria. Allows positive selection for integration. Replicates only in enterobacteria, and so functions as a suicide vector in other Gram negative hosts. When induced by media containing 5% sucrose, sacB is lethal in a wide range of Gram negative bacteria, and thus permits selection for loss of the vector. A single BglII site within the gentamicin resistance gene (gtmR) allows insertion of other resistance genes (e.g., as a BamHI cassette). The order of the major features of the plasmid is: gtmR - SstI/MCS/KpnI - P15A - traJ - oriT - sacB. [1] Deposited by: M.F.Hynes Grwoth media: LB plus gentamicin (ATCC medium number 1885) 37C Hosts: E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
产品说明书
PJQ200SK 载体序列 产品说明书(点击下载)
载体序列
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