back Return to this vector's summary. ID PSP64 preliminary; circular DNA; SYN; 2999 BP. XX AC X65327; L08943; VB0034; IG0094; XX DT 06-AUG-1987 (Rel. 2, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phagemid vector pSP64 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pSP64 RA ; RT ; RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by: RL Technical Services, Promega Corporation, 2800 Woods Hollow Road, RL Madison, WI 53711-5399, USA. XX RN [2] RP 1-2999 RC pSP64 RA Klock C.; RT ; RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by: RL Technical Services, Promega Corporation, 2800 Woods Hollow Road, RL Madison, WI 53711-5399, USA. XX RN [3] RC pSP64 from pUC12 & SP6 promoter RC pSP65 from pUC12 & SP6 promoter RA Melton D.A., Krieg P.A., Rebagliati M.R., Maniatis T., Zinn K., RA Green M.R.; RT "Efficient in vitro synthesis of biologically active RNA and RNA RT hybridization probes from plasmids containing a bacteriophage RT SP6 promoter"; RL Nucleic Acids Res. 12:7035-7056(1984). XX RN [4] RC pSP64 RA Melton D.A.; RT ; RL Submitted (00-APR-1986) to GenBank on computer-readable media by: RL Melton D.A., Harvard University. XX RN [5] RC pSP64 RA Gilbert W.; RT "Obtained from VecBase 3.0"; RL Unpublished (1991). XX RN [6] RC pSP6-HbetaA from pSP6-Hbeta RC pSP6-HbetaoFA from pSP6-HbetaA & piSVHPbetao RC pSP62-PL1 from human beta-globin gene RA Green M.R., Maniatis T., Melton D.A.; RT "Human beta-globin pre-mRNA synthesized in vitro is accurately RT spliced in Xenopus oocyte nuclei"; RL Cell 32:681-694(1983). XX RN [7] RC R7delta7 from pBR322 & SP6 promoter RC pSP6-Hbeta from R7delta7 & human beta-globin gene RA Butler E., Little P.; RT ; RL Unpublished (1983). XX RN [8] RC piSVHPbetao from beta0-thallassemic gene RA Treisman R., Proudfoot N.J., Shander M., Maniatis T.; RT "A single base change at a splice site in a beta0-thallassemic gene RT causes abnormal RNA splicing"; RL Cell 29:903-911(1982). XX CC During the construction of the pSP64 vector from pUC12, the CC majority of the lacZ gene was deleted. Therefore. the X-gal CC color screening method for the identification of recombinants is CC not possible with this vector. CC old Boehringer vector. CC NM (pSP64) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (phagemid) CC SP (Promega) CC HO (E.coli HB101)(E.coli LE392)(E.coli JM83) CC CP (high) CC FN (transcription) CC SE (color blue/white) CC PA (pUC12)(bGal12)(PromSP6)(pBR322) CC BR (pSP65) CC OF (pGEM-1)(pCKSP6) CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. pUC12 remove small FnuDII-Sau3A/MboI, FT \ 2680bp- FT \ MboI = 249 399 984 1020 1037 1295 1341 1359 1700 FT \ 1805 1817 1895 1903 1914 1989 FT \ FnuDII = 30 674 716 1108 FT 2. Sp6 FnuDII-Sau3A/MboI 400bp, promoter FT -> pSP64 2999bp [no lacZ]" FT promoter 1..1 FT /note="PRO Salmonella bacteriophage Sp6 FT transcription initiation site" FT misc_binding 7..57 FT /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI- FT EcoRI, from M13mp10/pUC12" FT misc_feature 57..286 FT /note="E. coli lac operon" FT misc_feature 58..2288 FT /note="from pUC12 bases 450..2680" FT misc_feature 71..71 FT /note="E. coli lacZ gene transcription initiation FT site" FT promoter 91..107 FT /note="PRO E. coli lac operator" FT misc_binding 232..232 FT /note="SIT unique PvuII" FT misc_binding 408..408 FT /note="SIT unique AflIII" FT rep_origin <408..<1000 FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT CDS 1228..2088 FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" FT misc_binding 1421..1421 FT /note="SIT unique BglI" FT misc_binding 1523..1523 FT /note="SIT unique AvlII" FT misc_binding 1671..1671 FT /note="SIT unique PvuII" FT misc_binding 1781..1781 FT /note="SIT unique ScaI" FT misc_binding 1900..1900 FT /note="SIT unique Asp700I" FT misc_binding 2105..2105 FT /note="SIT unique SspI" FT misc_binding 2223..2223 FT /note="SIT unique AatII" FT misc_feature 2289..2475 FT /note="from pUC12 bases 1..187" FT misc_feature 2522..2800 FT /note="from pBR322 bases complement(376..654)" FT misc_binding 2567..2567 FT /note="SIT unique SphI" FT misc_binding 2597..2597 FT /note="SIT unique DsaI" FT misc_binding 2633..2633 FT /note="SIT unique Eco47III" FT misc_binding 2726..2726 FT /note="SIT unique NaeI" FT misc_binding 2757..2757 FT /note="SIT unique NheI" FT misc_feature 2801..3045 FT /note="from bacteriophage Sp6" FT promoter join(2983..2999,1..6) FT /note="PRO bacteriophage Sp6; from Salmonella " XX SQ Sequence 2999 BP; 744 A; 772 C; 761 G; 722 T; 0 other; gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata //