File: pCoHYGRO 24-0436 Mode: Normal 1 - 4526 Table: DNASIS AVAIL Style: Circular Indication Mode: Actual Cutting Site Enzyme Name Sequence Count Cutting Position AatII GACGT!C 2 833 4462 Acc65I G!GTACC 1 2280 AccI GT!MKAC 1 419 AflII C!TTAAG 0 AflIII A!CRYGT 1 2647 AgeI A!CCGGT 0 AluI AG!CT 28 45 64 309 402 795 886 918 960 1345 1570 1635 1664 1854 1961 2135 2139 2159 2288 2312 2407 2471 2589 2815 2951 3208 3729 3829 3892 AlwI GGATCNNNN! 7 438 794 1585 2282 3296 3393 4173 AlwI !NNNNNGATCC 10 425 781 1265 1692 2065 3208 3294 3392 3856 4177 AlwNI CAGNNN!CTG 1 3063 ApaI GGGCC!C 0 ApaLI G!TGCAC 5 178 1086 1388 2961 4207 ApoI R!AATTY 3 1049 2194 2292 AscI GG!CGCGCC 0 AseI AT!TAAT 3 2418 2477 3712 AvaI C!YCGRG 2 2055 2125 AvaII G!GWCC 5 1214 1755 2023 3678 3900 AvrII C!CTAGG 0 BamHI G!GATCC 2 430 786 BanI G!GYRCC 5 236 1380 2280 2391 3488 BanII GRGCY!C 1 2290 BbsI !GAAGAC 0 BbsI !GTCTTC 0 BbvI GCAGCNNNNNNNN! 10 54 895 1153 1546 1673 2168 3066 3069 3275 3969 BbvI !NNNNNNNNNNNNGCTGC 9 242 315 947 1159 2458 2539 2557 2976 3578 BclI T!GATCA 0 BfaI C!TAG 7 425 1894 2136 2229 3142 3395 3730 BglI GCCNNNN!NGGC 2 252 3660 BglII A!GATCT 0 BpmI !CTGGAG 2 1452 1506 BpmI !CTCCAG 1 3624 Bpu1102I GC!TNAGC 1 1957 BsaA1 YAC!GTR 0 BsaB1 GATNN!NNATC 0 BsaH1 GR!CGYC 5 237 830 1798 4077 4459 BsaI !NNNNNGAGACC 1 3601 BsaI GGTCTCN! 0 BsaJI C!CNNGG 11 355 804 1158 1359 1514 1583 1814 2027 2028 2386 2807 BsgI GTGCAG! 1 2118 BsgI !CTGCAC 0 BsiEI CGRY!CG 12 280 1014 1149 1170 1179 1749 1844 1991 2563 2987 3910 4059 BsiWI C!GTACG 0 BslI CCNNNNN!NNGG 14 54 440 796 1027 1090 1377 1486 1707 2081 2495 2669 2687 2853 3132 BsmAI !GTCTC 3 868 4371 4524 BsmAI !GAGAC 2 51 3606 BsmFI !NNNNNGTCCC 1 2018 BsmFI GGGACN! 1 1714 BsmI GAATGCN! 0 BsmI G!CATTC 1 2224 Bsp1286I GDGCH!C 8 182 1090 1392 2035 2290 2965 4126 4211 BspDI AT!CGAT 0 BspEI T!CCGGA 3 1027 1564 1700 BspHI T!CATGA 3 3367 4375 4480 BspMI ACCTGCNNNN! 1 1119 BspMI !NNNNNNNNGCAGGT 1 405 BsrBI CCG!CTC 1 2339 BsrBI GAG!CGG 4 1197 1555 2580 4381 BsrDI !CATTGC 1 3775 BsrDI GCAATGNN! 1 3601 BsrFI R!CCGGY 3 1144 1986 3620 BsrGI T!GTACA 0 BsrI ACTGGN! 5 1292 1457 3055 3068 4016 BsrI C!CAGT 8 366 392 2447 3180 3586 3704 3747 4186 BssHII G!CGCGC 0 Bst1107I GTA!TAC 0 BstBI TT!CGAA 0 BstEII G!GTNACC 0 BstNI CC!WGG 6 356 2029 2387 2675 2796 2809 BstPI G!GTNACC 0 BstUI CG!CG 22 4 6 109 827 1016 1151 1262 1328 1394 1543 1585 1745 1886 1888 1978 2494 2496 2694 3275 3605 4098 4430 BstXI CCANNNNN!NTGG 0 BstYI R!GATCY 8 430 786 3288 3299 3385 3397 4165 4182 Bsu36I CC!TNAGG 0 ClaI AT!CGAT 0 DdeI C!TNAG 10 172 816 1184 1871 1957 2922 3331 3497 4037 4463 DpnI GA!TC 28 279 432 788 851 984 1169 1182 1272 1579 1699 2063 2072 2088 2119 2276 3215 3290 3301 3309 3387 3399 3504 3845 3863 3909 4167 4184 4220 DpnII !GATC 28 277 430 786 849 982 1167 1180 1270 1577 1697 2061 2070 2086 2117 2274 3213 3288 3299 3307 3385 3397 3502 3843 3861 3907 4165 4182 4218 DraI TTT!AAA 3 3406 3425 4117 DraIII CACNNN!GTG 2 1094 1387 DrdI GACNNNN!NNGTC 4 98 1310 1691 2755 DsaI C!CRYGG 4 804 1158 1514 1583 EaeI Y!GGCCR 9 389 568 798 1011 1176 1429 1746 2486 3928 EagI C!GGCCG 3 1011 1176 1746 Eam1105I GACNNN!NNGTC 1 3540 EarI !CTCTTC 3 290 2524 4328 EarI !GAAGAG 0 Eco47III AGC!GCT 0 Eco57I !CTGAAG 1 3173 Eco57I !CTTCAG 1 4221 EcoNI CCTNN!NNNAGG 0 EcoO109I RG!GNCCY 2 2004 4516 EcoRI G!AATTC 2 1049 2292 EcoRII !CCWGG 6 354 2027 2385 2673 2794 2807 EcoRV GAT!ATC 0 Esp3I !NNNNNGAGACG 1 46 Esp3I CGTCTCN! 2 874 4 Fnu4HI GC!NGC 34 43 152 256 329 884 961 964 1014 1142 1173 1176 1432 1535 1583 1586 1662 1746 2092 2157 2472 2553 2571 2574 2692 2847 2990 3055 3058 3264 3592 3931 3958 4053 4282 FokI GGATGNNNNNNNNN! 3 91 335 1175 FokI !NNNNNNNNNNNNNCATCC 4 1548 3506 3687 3974 FspI TGC!GCA 2 259 3762 HaeII RGCGC!Y 3 240 2525 2895 HaeIII GG!CC 25 289 391 570 800 1013 1157 1178 1206 1358 1431 1513 1748 1919 1986 2005 2131 2488 2662 2673 2691 3125 3583 3663 3930 4517 HgaI GACGCNNNNN! 5 1549 1694 1806 2758 3336 HgaI !NNNNNNNNNNGCGTC 4 98 856 1308 4066 HgiAI GWGCW!C 7 182 1090 1392 2290 2965 4126 4211 HhaI GCG!C 24 6 109 239 260 1018 1262 1330 1545 1672 1745 1888 2431 2496 2524 2557 2827 2894 2994 3168 3277 3670 3763 4100 4432 HincII GTY!RAC 2 420 1642 HindIII A!AGCTT 1 400 HinfI G!ANTC 11 421 902 1024 1224 1475 1998 2482 2547 2622 3018 3535 HpaI GTT!AAC 0 HpaII C!CGG 21 49 83 434 1028 1145 1378 1565 1591 1701 1707 1987 2365 2854 3001 3027 3217 3621 3655 3722 3832 4074 HphI GGTGANNNNNNNN! 5 25 34 2071 4026 4267 HphI !NNNNNNNTCACC 3 3383 3610 4232 KasI G!GCGCC 1 236 KpnI GGTAC!C 1 2284 MaeII A!CGT 9 375 534 641 830 1100 3350 3766 4139 4459 MaeIII !GTNAC 13 57 348 368 1096 2168 3003 3066 3182 3465 3796 3854 4007 4195 MboI !GATC 28 277 430 786 849 982 1167 1180 1270 1577 1697 2061 2070 2086 2117 2274 3213 3288 3299 3307 3385 3397 3502 3843 3861 3907 4165 4182 4218 MboII GAAGANNNNNNNN! 2 911 3309 MboII !NNNNNNNTCTTC 8 284 1492 1495 2518 3380 4135 4213 4322 MluI A!CGCGT 0 MnlI !NNNNNNNGAGG 18 420 885 922 1146 1353 1452 1479 1502 1550 1808 1974 2120 2495 2754 3078 3478 3559 4506 MnlI CCTCNNNNNNN! 10 40 300 502 1394 1880 2134 2546 2829 3707 3913 MscI TGG!CCA 2 570 800 MseI T!TAA 16 143 340 537 692 744 2418 2477 3353 3405 3410 3424 3477 3712 3751 4116 4488 MspI C!CGG 21 49 83 434 1028 1145 1378 1565 1591 1701 1707 1987 2365 2854 3001 3027 3217 3621 3655 3722 3832 4074 MunI C!AATTG 0 NaeI GCC!GGC 0 NarI GG!CGCC 1 237 NciI CC!SGG 7 49 84 1592 1708 3027 3723 4074 NcoI C!CATGG 2 804 1158 NdeI CA!TATG 2 185 1256 NgoMI G!CCGGC 0 NheI G!CTAGC 1 2135 NlaIII CATG! 18 42 410 454 808 1162 1245 1280 2046 2270 2306 2651 3371 3862 3872 3950 3986 4379 4484 NlaIV GGN!NCC 18 238 432 488 788 1382 1405 1589 1705 2025 2282 2393 2679 2718 3490 3584 3625 3836 4426 NotI GC!GGCCGC 0 NruI TCG!CGA 0 NsiI ATGCA!T 0 Nsp(7524)I RCATG!Y 3 42 410 2651 NspBII CMG!CKG 9 115 309 1133 1442 1585 2471 2989 3234 4175 PacI TTAAT!TAA 0 PflMI CCANNNN!NTGG 0 PleI GAGTCNNNN! 1 3026 PleI !NNNNNGACTC 3 415 2541 3529 PmeI GTTT!AAAC 0 PmlI CAC!GTG 0 Psp1406I AA!CGTT 3 534 3766 4139 PstI CTGCA!G 2 416 1143 PvuI CGAT!CG 3 280 1170 3910 PvuII CAG!CTG 2 309 2471 RsaI GT!AC 7 170 640 823 1723 1776 2282 4020 RsrII CG!GWCCG 1 1214 SacI GAGCT!C 1 2290 SacII CCGC!GG 1 1586 SalI G!TCGAC 1 418 SapI !NNNNGAAGAGC 0 SapI GCTCTTCN! 1 2531 Sau3AI !GATC 28 277 430 786 849 982 1167 1180 1270 1577 1697 2061 2070 2086 2117 2274 3213 3288 3299 3307 3385 3397 3502 3843 3861 3907 4165 4182 4218 Sau96I G!GNCC 13 287 1205 1214 1356 1755 1984 2004 2023 3582 3661 3678 3900 4516 ScaI AGT!ACT 2 1776 4020 ScrFI CC!NGG 13 49 84 356 1592 1708 2029 2387 2675 2796 2809 3027 3723 4074 SfaNI GCATCNNNNN! 9 163 239 1016 1083 1570 2196 2744 3796 4236 SfaNI !NNNNNNNNNGATGC 9 69 199 870 1153 1338 1648 1670 1826 3987 SfcI C!TYRAG 5 2125 2753 3015 3292 4160 SfiI GGCCNNNN!NGGCC 0 SfrI !GCCCGGGC 0 SgrAI CR!CCGGYG 0 SmaI CCC!GGG 0 SnaBI TAC!GTA 0 SpeI A!CTAGT 0 SphI GCATG!C 1 410 SrfI GCCC!GGGC 0 Sse8387I CCTGCA!GG 1 416 SspI AAT!ATT 1 4344 StuI AGG!CCT 0 StyI C!CWWGG 2 804 1158 SwaI ATTT!AAAT 0 TaqI T!CGA 14 419 837 852 861 921 1338 1551 1653 1678 1861 2126 2290 2747 4191 TfiI G!AWTC 7 902 1024 1224 1475 1998 2482 2622 Tth111I GACN!NNGTC 2 866 1310 XbaI T!CTAGA 1 424 XcmI !CCANNNNNNNNNTGG 0 XhoI C!TCGAG 1 2125 XmaI C!CCGGG 0 XmaIII C!GGCCG 3 1011 1176 1746 XmnI GAANN!NNTTC 1 4139