File: pAc5.1/V5-His/lacZ 24-0576 Mode: Normal 1 - 8433 Table: DNASIS# Style: Circular Indication Mode: Actual Cutting Site Enzyme Name Sequence Count Cutting Position AatI AGG!CCT 1 5830 AatII GACGT!C 5 524 986 1569 3257 8370 Acc65I G!GTACC 1 2580 AccI GT!MKAC 3 1210 5398 6103 AccIII T!CCGGA 0 AcyI GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 AfaI GT!AC 13 231 1099 1887 2118 2271 2582 3338 3817 4129 4715 5407 5763 7928 AflI G!GWCC 7 2258 2468 4175 6250 6338 7586 7808 AflII C!TTAAG 1 1935 AflIII A!CRYGT 8 169 596 2113 3936 4716 5141 5393 5758 AgeI A!CCGGT 1 5764 AhaI CC!SGG 11 427 428 2571 4069 4159 4518 4695 4755 6935 7631 7982 AhaII GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 AhaIII TTT!AAA 6 1170 5794 6055 7314 7333 8025 Alw44I G!TGCAC 4 4979 5094 6869 8115 AlwI GGATCNNNN! 10 2581 2600 4051 4343 5384 5867 6556 7204 7301 8081 AlwI !NNNNNGATCC 11 2587 2635 3901 4051 4215 5854 7116 7202 7300 7764 8085 AlwNI CAGNNN!CTG 5 1577 4344 4769 5520 6971 AocI CC!TNAGG 1 2858 AocII GDGCH!C 10 2590 4574 4760 4983 5098 5623 5838 6873 8034 8119 Aor51HI AGC!GCT 3 2438 4469 6228 AosI TGC!GCA 3 1681 2776 7670 AosII GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 ApaI GGGCC!C 0 ApaLI G!TGCAC 4 4979 5094 6869 8115 ApoI R!AATTY 7 508 581 2329 2615 3130 5638 5939 ApyI CC!WGG 13 385 2678 2805 3109 4202 4770 5037 5071 5616 6511 6583 6704 6717 AquI C!YCGRG 4 426 3972 5415 5699 AscI GG!CGCGCC 0 AseI AT!TAAT 2 12 7620 AsnI AT!TAAT 2 12 7620 Asp700I GAANN!NNTTC 4 13 802 1873 8047 Asp718I G!GTACC 1 2580 AspHI GWGCW!C 8 2590 4574 4983 5098 5838 6873 8034 8119 AspI GACN!NNGTC 0 AsuII TT!CGAA 1 5714 AvaI C!YCGRG 4 426 3972 5415 5699 AvaII G!GWCC 7 2258 2468 4175 6250 6338 7586 7808 AvaIII ATGCA!T 1 1407 AviII TGC!GCA 3 1681 2776 7670 AvrI !CYCGRG 4 425 3971 5414 5698 AvrII C!CTAGG 0 AxyI CC!TNAGG 1 2858 BalI TGG!CCA 2 181 348 BamHI G!GATCC 2 2592 5859 BanI G!GYRCC 8 1389 2580 2814 2826 3430 3882 6217 7396 BanII GRGCY!C 4 2590 4574 5623 5838 BanIII AT!CGAT 2 1709 3458 BbeI GGCGC!C 1 1393 BbiII/AcyI GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 BbrPI CAC!GTG 0 BbsI !NNNNNNGTCTTC 4 254 5403 6181 8426 BbsI GAAGACNN! 1 4151 BbvI !NNNNNNNNNNNNGCTGC 14 127 1828 2449 3173 3251 3632 3635 4946 5422 5534 5965 6884 7486 7675 BbvII GAAGACNN! 1 4151 BbvII !NNNNNNGTCTTC 4 254 5403 6181 8426 BcefI ACGGCNNNNNNNNNNNN! 14 1338 1871 3120 3216 3594 3672 3852 3891 4029 4118 4380 4401 5114 7057 BcefI !NNNNNNNNNNNNNGCCGT 6 1111 1901 2941 3109 3649 3764 BclI T!GATCA 5 1821 3980 5803 5842 6096 BcnI CC!SGG 11 427 428 2571 4069 4159 4518 4695 4755 6935 7631 7982 BfaI C!TAG 9 309 1085 1960 1975 2599 5906 7050 7303 7638 BfrI C!TTAAG 1 1935 BglI GCCNNNN!NGGC 4 2786 4907 6386 7568 BglII A!GATCT 1 133 BinI !NNNNNGATCC 11 2587 2635 3901 4051 4215 5854 7116 7202 7300 7764 8085 BinI GGATCNNNN! 10 2581 2600 4051 4343 5384 5867 6556 7204 7301 8081 BlnI C!CTAGG 0 BmyI GDGCH!C 10 2590 4574 4760 4983 5098 5623 5838 6873 8034 8119 Bpu1102I GC!TNAGC 2 2440 5647 BsaAI YAC!GTR 0 BsaBI GATNN!NNATC 3 1770 3965 6095 BsaHI GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 BsaI GGTCTCN! 1 5752 BsaI !NNNNNGAGACC 2 2559 7509 BsaJI C!CNNGG 13 426 427 2262 2569 2676 2966 3873 4011 4100 4372 5036 5742 6715 BseAI T!CCGGA 0 BsePI !GCGCGC 1 4131 BsiWI C!GTACG 2 1097 5405 BsmAI !NNNNNGAGAC 4 1540 2559 4201 7509 BsmAI GTCTCN! 7 483 1045 1662 1674 3261 5752 8285 BsmI NG!CATTC 1 6008 BsmI GAATGCN! 4 285 504 1407 5915 Bsp1286I GDGCH!C 10 2590 4574 4760 4983 5098 5623 5838 6873 8034 8119 BspDI AT!CGAT 2 1709 3458 BspEI T!CCGGA 0 BspHI T!CATGA 4 56 7275 8283 8388 BspMI ACCTGCNNNN! 0 BspMI !NNNNNNNNGCAGGT 3 3408 5280 6505 BspMII T!CCGGA 0 BsrFI R!CCGGY 9 1052 1330 1930 3921 5653 5764 6220 6380 7528 BsrI NC!CAGT 15 2316 2471 2604 4419 4503 4902 5469 5487 5670 6131 7088 7494 7612 7655 8094 BsrI ACTGGN! 12 349 2671 2893 3344 4282 4333 4585 5314 6340 6963 6976 7924 BssHII G!CGCGC 1 4132 Bst1107I GTA!TAC 1 5399 BstBI TT!CGAA 1 5714 BstEII G!GTNACC 1 5704 BstI G!GATCC 2 2592 5859 BstNI CC!WGG 13 385 2678 2805 3109 4202 4770 5037 5071 5616 6511 6583 6704 6717 BstPI G!GTNACC 1 5704 BstXI CCANNNNN!NTGG 3 2611 4854 5471 BstYI R!GATCY 11 133 2592 2640 5376 5859 7196 7207 7293 7305 8073 8090 Bsu36I CC!TNAGG 1 2858 CbiI TT!CGAA 1 5714 CcrI C!TCGAG 1 5699 CelII GC!TNAGC 2 2440 5647 Cfr10I R!CCGGY 9 1052 1330 1930 3921 5653 5764 6220 6380 7528 Cfr9I C!CCGGG 1 426 CfrI Y!GGCCR 8 179 346 1372 3105 4103 5693 6390 7836 ClaI AT!CGAT 2 1709 3458 CpoI CG!GWCCG 0 Csp45I TT!CGAA 1 5714 CspI CG!GSCCG 0 CvnI CC!TNAGG 1 2858 DraI TTT!AAA 6 1170 5794 6055 7314 7333 8025 DraII RG!GNCCY 2 2258 8424 DraIII CACNNN!GTG 1 3824 DrdI GACNNNN!NNGTC 2 4743 6663 DsaI C!CRYGG 6 2262 2966 3873 4011 4100 4372 EaeI Y!GGCCR 8 179 346 1372 3105 4103 5693 6390 7836 EagI C!GGCCG 2 5693 6390 Eam1105I GACNNN!NNGTC 1 7448 EarI !NNNNGAAGAG 1 2735 EarI CTCTTCN! 4 488 1669 2281 8243 Ecl136II GAG!CTC 3 2588 4572 5836 EclXI C!GGCCG 2 5693 6390 Eco105I TAC!GTA 0 Eco31I GGTCTCN! 1 5752 Eco31I !NNNNNGAGACC 2 2559 7509 Eco47I G!GWCC 7 2258 2468 4175 6250 6338 7586 7808 Eco47III AGC!GCT 3 2438 4469 6228 Eco52I C!GGCCG 2 5693 6390 Eco81I CC!TNAGG 1 2858 EcoNI CCTNN!NNNAGG 0 EcoO109I RG!GNCCY 2 2258 8424 EcoO65I G!GTNACC 1 5704 EcoRI G!AATTC 2 2615 5638 EcoRII !CCWGG 13 383 2676 2803 3107 4200 4768 5035 5069 5614 6509 6581 6702 6715 EcoRV GAT!ATC 1 3747 EcoT14I C!CWWGG 1 2262 EcoT22I ATGCA!T 1 1407 EcoT38I GRGCY!C 4 2590 4574 5623 5838 EheI GGC!GCC 1 1391 Esp3I CGTCTCN! 3 1662 1674 3261 Esp3I !NNNNNGAGACG 2 1540 4201 EspI GC!TNAGC 2 2440 5647 FbaI T!GATCA 5 1821 3980 5803 5842 6096 FokI GGATGNNNNNNNNN! 9 3246 3736 3865 4153 4596 4609 4645 6451 6496 FokI !NNNNNNNNNNNNNCATCC 9 2696 3686 3791 4448 4816 5240 7414 7595 7882 FspI TGC!GCA 3 1681 2776 7670 GdiII Y!GGCCG 4 1372 5693 6390 7836 GdiII C!GGCCR 4 3105 4103 5693 6390 HaeI WGG!CCW 11 181 348 3032 4010 5270 5830 6371 6443 6500 6581 7033 HaeII RGCGC!Y 14 1091 1393 2440 2799 3093 3530 4019 4471 4594 4694 4769 5654 6230 6803 HgaI GACGCNNNNN! 10 3045 4036 4218 4815 4962 5451 6404 6436 6666 7244 HgaI !NNNNNNNNNNGCGTC 8 248 743 1128 1644 3474 4771 4808 7974 HgiAI GWGCW!C 8 2590 4574 4983 5098 5838 6873 8034 8119 HgiEII !ACCNNNNNNGGT 2 2816 7135 Hin1I GR!CGYC 10 521 983 1390 1566 1655 3254 4782 4954 7985 8367 HincII GTY!RAC 8 633 1185 3060 3684 5512 5994 6104 7989 HindIII A!AGCTT 0 HinfI G!ANTC 15 76 444 1508 2236 2973 3540 3672 3891 4003 5611 5752 6303 6457 6926 7443 HpaI GTT!AAC 3 3060 3684 5994 HphI GGTGANNNNNNNN! 7 3189 3421 3460 4402 4630 7934 8175 HphI !NNNNNNNTCACC 10 410 978 3961 4965 5698 5767 5773 7291 7518 8140 KasI G!GCGCC 1 1389 KpnI GGTAC!C 1 2584 Ksp632I CTCTTCN! 4 488 1669 2281 8243 Ksp632I !NNNNGAAGAG 1 2735 KspI CCGC!GG 0 MaeI C!TAG 9 309 1085 1960 1975 2599 5906 7050 7303 7638 MamI GATNN!NNATC 3 1770 3965 6095 MboII GAAGANNNNNNNN! 9 507 550 1593 1990 2752 3226 4156 5569 7217 MboII !NNNNNNNTCTTC 13 254 475 1656 2268 2845 5403 6181 6452 7288 8043 8121 8230 8426 MfeI !CAATTG 2 1417 6002 MflI R!GATCY 11 133 2592 2640 5376 5859 7196 7207 7293 7305 8073 8090 MluI A!CGCGT 4 3936 4716 5141 5758 MroI T!CCGGA 0 MscI TGG!CCA 2 181 348 MstI TGC!GCA 3 1681 2776 7670 MstII CC!TNAGG 1 2858 MunI C!AATTG 2 1418 6003 MvaI CC!WGG 13 385 2678 2805 3109 4202 4770 5037 5071 5616 6511 6583 6704 6717 NaeI GCC!GGC 2 6222 6382 NarI GG!CGCC 1 1390 NciI CC!SGG 11 427 428 2571 4069 4159 4518 4695 4755 6935 7631 7982 NcoI C!CATGG 1 2262 NdeI CA!TATG 2 2121 5591 NgoMI G!CCGGC 2 6220 6380 NheI G!CTAGC 0 NotI GC!GGCCGC 1 5693 NruI TCG!CGA 2 515 6425 NsiI ATGCA!T 1 1407 Nsp(7524)I RCATG!Y 8 600 779 1106 1122 1640 2117 2245 5397 Nsp(7524)V TT!CGAA 1 5714 NspII GDGCH!C 10 2590 4574 4760 4983 5098 5623 5838 6873 8034 8119 NspIII C!YCGRG 4 426 3972 5415 5699 NunII GG!CGCC 1 1390 PacI TTAAT!TAA 0 PaeR71 C!TCGAG 1 5699 PflMI CCANNNN!NTGG 3 384 3879 4245 PleI GAGTCNNNN! 1 6934 PleI !NNNNNGACTC 3 438 5605 7437 PmaCI CAC!GTG 0 PmeI GTTT!AAAC 1 5794 PmlI CAC!GTG 0 PpuMI RG!GWCCY 1 2258 PshAI GACNN!NNGTC 1 6167 PstI CTGCA!G 2 2624 7693 PvuI CGAT!CG 7 392 2757 3483 3963 4416 5855 7818 PvuII CAG!CTG 5 1907 2203 2726 5283 5646 RmaI C!TAG 9 309 1085 1960 1975 2599 5906 7050 7303 7638 RsaI GT!AC 13 231 1099 1887 2118 2271 2582 3338 3817 4129 4715 5407 5763 7928 RsrI G!AATTC 2 2615 5638 RsrII CG!GWCCG 0 SacI GAGCT!C 3 2590 4574 5838 SacII CCGC!GG 0 SalI G!TCGAC 1 6102 SauI CC!TNAGG 1 2858 ScaI AGT!ACT 1 7928 SduI GDGCH!C 10 2590 4574 4760 4983 5098 5623 5838 6873 8034 8119 SecI C!CNNGG 13 426 427 2262 2569 2676 2966 3873 4011 4100 4372 5036 5742 6715 SexI !CTCGAG 1 5698 SfaNI GCATCNNNNN! 12 74 1867 3705 4470 4845 4874 5160 5262 6486 6652 7704 8144 SfaNI !NNNNNNNNNGATGC 11 82 719 1799 1955 2885 2898 5106 5937 6099 6474 7895 SfcI C!TRYAG 7 605 2620 3022 5505 6820 7011 7689 SfiI GGCCNNNN!NGGCC 0 SfuI TT!CGAA 1 5714 SgrAI CR!CCGGYG 0 SinI G!GWCC 7 2258 2468 4175 6250 6338 7586 7808 SmaI CCC!GGG 1 428 SnaBI TAC!GTA 0 SnaI !GTATAC 1 5396 SnoI G!TGCAC 4 4979 5094 6869 8115 SpeI A!CTAGT 2 1959 2598 SphI GCATG!C 1 1106 SplI C!GTACG 2 1097 5405 SrfI GCCC!GGGC 0 Sse8387I CCTGCA!GG 0 SspI AAT!ATT 5 1434 1735 2054 3864 8252 SstI GAGCT!C 3 2590 4574 5838 SstII CCGC!GG 0 StuI AGG!CCT 1 5830 StyI C!CWWGG 1 2262 StySJI !GAGNNNNNNGTRC 0 StySJI !GYACNNNNNNCTC 2 94 1885 SwaI ATTT!AAAT 0 TfiI G!AWTC 11 76 1508 2236 2973 3540 3672 3891 4003 5752 6303 6457 Tth111I GACN!NNGTC 0 Tth111II CAARCANNNNNNNNNNN! 5 222 1610 2319 7145 7184 Tth111II !NNNNNNNNNTGYTTG 5 1288 1439 1631 1665 7152 Van91I CCANNNN!NTGG 3 384 3879 4245 VspI AT!TAAT 2 12 7620 XbaI T!CTAGA 0 XcmI CCANNNNN!NNNNTGG 2 4851 5596 XcyI C!CCGGG 1 426 XhoI C!TCGAG 1 5699 XhoII R!GATCY 11 133 2592 2640 5376 5859 7196 7207 7293 7305 8073 8090 XmaI C!CCGGG 1 426 XmaIII C!GGCCG 2 5693 6390 XmnI GAANN!NNTTC 4 13 802 1873 8047 XorII CGAT!CG 7 392 2757 3483 3963 4416 5855 7818