Binary vector pCAMBIA-2300, complete sequence GenBank: AF234315.1 FeaturesSequenceLOCUS AF234315 8742 bp DNA circular SYN 24-APR-2000 DEFINITION Binary vector pCAMBIA-2300, complete sequence. ACCESSION AF234315 VERSION AF234315.1 GI:7638145 KEYWORDS . SOURCE Binary vector pCAMBIA-2300 ORGANISM Binary vector pCAMBIA-2300 other sequences; artificial sequences; vectors. REFERENCE 1 (sites) AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P. TITLE The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994) PUBMED 7919218 REFERENCE 2 (bases 1 to 8742) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE A comprehensive set of modular vectors for advanced manipulations and efficient transformation of plants JOURNAL Unpublished REMARK Full description of constructs REFERENCE 3 (bases 1 to 8742) AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W., Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L., Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z., Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A. TITLE Direct Submission JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain / GPO Box 3200, Canberra, ACT 2601, Australia FEATURES Location/Qualifiers source 1..8742 /organism="Binary vector pCAMBIA-2300" /mol_type="other DNA" /db_xref="taxon:118407" misc_feature complement(980..1980) /note="STA region from pVS1 plasmid" rep_origin complement(2573..3573) /note="pVS1-REP; replication origin from pVS1" misc_feature complement(3983..4243) /note="bom site from pBR322" rep_origin complement(4383..4663) /note="pBR322 origin of replication" CDS complement(4954..5748) /note="aadA (kanamycin resistance) gene amplified from pIG121Hm" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAF65399.1" /db_xref="GI:7638147" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 6173..6198 /note="left border repeat from C58 T-DNA" misc_feature 6265..6468 /note="CaMV 3'UTR (polyA signal)" CDS complement(6506..7303) /note="nptII (kanamycin resistance) gene" /codon_start=1 /product="neomycin phosphotransferase" /protein_id="AAF65400.1" /db_xref="GI:7638148" /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(7334..8114) /note="CaMV35S2; CaMV 35S promoter, duplicated" promoter 8262..8316 /note="PlacZ; lacZ promoter" CDS 8344..8577 /codon_start=1 /product="LacZ alpha fragment" /protein_id="AAF65398.1" /db_xref="GI:7638146" /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC" misc_feature 8358..8414 /note="pUC18 MCS; polylinker" misc_feature 8656..8681 /note="right border T-DNA repeat" ORIGIN 1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata 4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata 5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt 5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa 5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt 5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc 5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc 5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc 5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag 5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag 5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac 5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc 5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta 5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct 5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc 5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg 5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca 5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg 6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag 6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg 6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg 6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga 6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc 6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca 6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg 6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga 6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat 6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt 6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg 6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca 7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc 7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg 7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg 7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag 7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct 7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc 7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg 7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga 7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg 7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca 7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg 7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact 7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa 7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt 7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta 7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga 7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc 8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg 8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc 8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg 8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta 8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca 8341 gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 8401 aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 8461 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 8521 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc 8581 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac 8641 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta 8701 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tg //